All NAPI core facilities have extensive experience in protein identification by mass spectrometry.
Users can submit a sample of interest (e.g. protein complex, immunoprecipitation etc) to one of our core facilities, and our highly sensitive MS instruments can generate a list of proteins present within sample, even those present at very low levels.
In a typical experimental set-up, proteins in the sample are digested using proteolytic enzymes such as trypsin, and the resulting mixtures of peptides are separated by high performance liquid chromatrography. Tandem mass spectrometry (MS/MS) is then used to determine the amino acid sequence of the peptides, and bioinformatics pipelines are used to analyse the MS/MS data and generate a list of proteins contained within the sample.
In addition to protein identification based on peptide sequencing, we are also able to perform ‘top down’ protein identification, in which intact proteins are detected by MS, without prior digestion into peptides.
NAPI core facilities can provide expert assistance with all aspects of users' protein identification projects, including experimental design related to sample generation and follow-up data analysis pipelines.