The composition of these multi-protein complexes may change in response to specific stimuli, leading to downstream changes in cell behaviour (e.g. stimulation by growth factors to promote cell proliferation). Defining protein complexes and their dynamics is therefore fundamental principal of cell biology, and has implications for a range of disease states.
Large-scale analysis of protein complexes, or interactomics, is typically carried out by pull-down experiments with tagged proteins (bait) followed by MS identification of proteins interacting with the bait (AP-MS). Several NAPI partners have experience AP-MS and can provide expert assistance in detecting interaction partners for your protein of interest.
Additionally, NAPI has access to the APEX2 and/or BioID2 tag strategies for purification and MS-based identification of protein complexes. Please contact our administrative manager for more information on these techniques.
