Epigenetic/epitranscriptomic modifications in DNA and RNA play diverse and important roles in regulating cell behaviour. The systems-wide study of such modifications is referred to as modomics.
Modomics at NAPI
How and when genes are turned on and off is essential to human development and health. Gene expression is regulated by dynamic epigenetic modifications on chromatin proteins and DNA, as well as epitranscriptomic modifications on RNA. In the last few years many novel epigenetic/epitranscriptomic modifications in DNA and RNA have been described that are important to maintain cell and body homeostasis.
Researchers at the Proteomics and Modomics Experimental Core (PROMEC) at NTNU have extensive experience in analyzing epigenetic/epitranscriptomic modifications by mass spectrometry (MS). Researchers interested in performing modomics experiments can contact PROMEC or the NAPI administrative manager to discuss the needs for their project.
Through NAPI funding, PROMEC will soon install new chromatography equipment for the preparative isolation of specific RNA species prior to mass spectrometry analyses. This will facilitate highly accurate RNA modification profiling and thereby benefit a broad range of modomics projects.
Several novel proteins (“writers”, “erasers” and “readers”) associated with DNA/RNA modifications have recently been identified but are still poorly understood. Researchers at PROMEC are establishing a first-of-its-kind pipeline for parallel and highly sensitive quantification of writers, erasers and readers, as well as concurrent absolute quantification of the DNA/RNA modification events regulated by these proteins.