New paper in Cell: investigating methylation events that regulate RNA splicing

Cathrine Broberg Vågbø from the NAPI core facility at NTNU was involved in a collaborative study recently published in the journal Cell.

Graphical abstract shows an overview of the mechansims regulating splicing of sams-3 mRNA, which ultimately maintains stable SAM levels. Mendel et al. (2021)

This study investigated the N6-methyladenosine (m6A) RNA modification, which is known as a common mechanism that regulates the fate of mRNAs.

Researchers based at the University of Geneva, Switzerland, worked with Dr Vågbø at the Proteomics and Modomics Experimental Core (PROMEC) at NTNU, to gain better insight into the molecular mechanisms controlling mRNA splicing. Using the model organism C. elegans, they identified a key m6A event that, via regulation of mRNA splicing, blocks the production of a protein called S-adenosylmethionine (SAM) synthetase. As summarised in the graphical abstract to the right, the mechanism is triggered by a rich diet and acts as a switch to stop SAM synthetase production and regulate its levels in cells. This, in turn, maintains appropriate levels of SAM (produced by SAM synthetase), which is required for methylation reactions such as m6A. Thus the mechanism represents an important feedback loop whereby too much SAM under nutrient-rich conditions triggers m6A modifcations on SAM synthetase mRNA, reduced SAM synthetase levels and a subsequent reduction in SAM levels.

The researchers propose that use of splice-site m6A is an ancient mechanism for splicing regulation and likely relevant to many diverse species.

You can read the publication in full on the Cell website.

The PROMEC core facility possesses strong expertise in the field of modomics (using mass spectrometry to study modifications to DNA and RNA). You can learn more on the modomics section of the research and techniques pages of the NAPI website. 

 

Published Oct. 29, 2021 10:47 AM - Last modified Oct. 29, 2021 10:48 AM