New Orbitrap Eclipse for University of Bergen Proteomics Core Facility
The NAPI core facility at the University of Bergen (PROBE) recently received its newest mass spectrometer; the Orbitrap Eclipse from Thermo Scientific.
The Thermo Scientific Orbitrap Eclipse at UiB. Photo: PROBE/UiB
NAPI is funded by the Research Council of Norway, and officially launched in 2020. One of the first items on the NAPI agenda involves upgrading the instrumentation at all of the proteomics/mass spectrometry core facilities in Norway.
Expanding proteomic capabilities
The arrival of the Orbitrap Eclipse marks the second new instrument for NAPI core facilities, after the Orbitrap Exploris was installed at the Tromsø University Proteomics Platform in November. Both machines will expand the analytical capabilities of the respective core facilities, and thereby benefit a broad range of research projects within the Norwegian research community.
For example, the Orbitrap Eclipse represents a cutting-edge instrument that can perform a number of state-of-the-art analytical tasks not widely available at NAPI core facilities. These include techniques such three-stage mass spectrometry (MS3), in which fragmented peptide ions are subjected to an additional fragmentation event, enabling better sequence coverage and therefore improved peptide/protein identification and detection of post-translationally modified amino acids.
Future goals: single cell proteomics
Head of the PROBE core facility, Dr Frode Bergen, provides more insight into the benefits of the Eclipse:
“Compared to our other instruments, the Eclipse brings higher sensitivity, faster sequencing and more precise m/z measurements – factors that will in general improve the quality of the MS data we can now generate. Furthermore, the sensitivity afforded by the Eclipse is essential for the successful implementation of single cell proteomics. This is somewhat of a ‘holy grail’ in proteomics, but something we hope to achieve within the NAPI infrastructure. The sample preparation steps are major bottlenecks, but if sample loss is kept to a minimum we know the Eclipse is capabale of detecting a substantial number of proteins from single cells”.
The Eclipse is currently being installed at the PROBE facility, and will soon be ready for the first set of samples. We look forward to seeing the new data.
More new mass spectrometers will be installed at the other NAPI core facilities in the near future. Information about these instruments and the new research opportunities they bring will be shared on the NAPI website.