The Thermo Scientific Orbitrap Eclipse arrived at the Proteomics Unit at the University of Bergen (PROBE) in November 2020.
The Thermo Scientific Orbitrap Eclipse at UiB. Photo: PROBE/UiB
The Orbitrap Eclipse represents a cutting-edge instrument that can perform a number of state-of-the-art analytical tasks. These include:
- three-stage mass spectrometry (MS3), in which fragmented peptide ions are subjected to an additional fragmentation event, enabling better sequence coverage and therefore improved peptide/protein identification and detection of post-translationally modified amino acids.
- pre-set parameters/intelligence for workflows such as tandem mass tag (TMT), a chemical labeling technique for protein quantification.
- Fragmentation techniques such as electron transfer dissociation (ETD) to enable better sequence coverage and detection of modified residues (e.g. phosphorylated serine/threonine/tyrosine).
In general, the Eclipse makes use of the latest improvements in fundamental mass spectrometer parameters such as ion transmission efficiency, data acquisition speed, and mass range. Compared to other instruments at PROBE, the Eclipse facilitates enhanced sensitivity, selectivity and precision, and will benefit a broad range of research projects both within the University of Bergen and across the NAPI infrastructure.